Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor
A. Tiwari, S. K. Shukla
Vol. 3., No.9., Pages 553-559, 2009
DOI: 10.3144/expresspolymlett.2009.69
DOI: 10.3144/expresspolymlett.2009.69
Corresponding author: A. Tiwari 
GRAPHICAL ABSTRACT
ABSTRACT
A novel matrix composed of chitosan-graft-polyaniline (CHIT-g-PANI) was electrochemically prepared to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-g-PANI matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-g-PANI biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), contact angle measurement and cyclic voltammetry (CV) taking CHIT-g-PANI as a reference. The influence of various parameters on CAH enzyme activity within the matrix was investigated including pH, temperature, and time. The Michaelis-Menten constant and apparent activities for the CAH enzyme were calculated to be 0.51 mM and 83.59 mg/cm2, respectively; indicating CHIT-g-PANI matrix has a high affinity to immobilize CAH enzyme.
